Phospholipid distribution pattern in uninduced (resident) and casein-induced mouse peritoneal cells.

peritoneal exudate cells differ from the resident peritoneal cells obtained from unstimulated animals. The most characteristic difference between the two kinds is the •gstickiness" of the former cells as manifested by failure to migrate from a cell mass packed by centrifugation in a capillary tube (David et al., 1964; Bloom and Bennett, 1966). McLaughlin, Ruddle and Waksman (1972) reported that the stickiness and the cytopathic activity were related to the degree of activation of peritoneal exudate cells as measured by larger size, lower nucleocytoplasmic ratio, and increased acid phosphatase activity, and that these properties vary with the nature of the irritant used to obtain the exudate. A number of studies have been accumulated to confirm this fact. Stickiness appears to suggest some change of the surface properties of the cell, and we are interested in comparing the phospholipid patterns between the resident and casein-induced peritoneal cells of mice. Two hundred and thirty mice (ddY, male, 18 g) were divided into four